PlantTFDB
Plant Transcription Factor Database
v4.0
Previous version: v3.0
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID AT5G05660.1
Common NameATNFXL2, EBI, MJJ3.6, NFXL2
Organism
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Family NF-X1
Protein Properties Length: 880aa    MW: 98882.3 Da    PI: 8.3072
Description sequence-specific DNA binding transcription factors;zinc ion binding;sequence-specific DNA binding transcription factors
Gene Model
Gene Model ID Type Source Coding Sequence
AT5G05660.1genomeTAIRView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
1zf-NF-X116.42e-05247266120
     zf-NF-X1   1 CGkHkCqklCHeGpCppCpq 20 
                  C +H C ++CH G+CppC++
  AT5G05660.1 247 CNIHNCREICHDGECPPCRE 266
                  449***************85 PP

2zf-NF-X116.51.8e-05354373119
     zf-NF-X1   1 CGkHkCqklCHeGpCpp.Cp 19 
                  CG H+C + CH+GpC + C+
  AT5G05660.1 354 CGYHRCPERCHRGPCLEtCR 373
                  *****************997 PP

3zf-NF-X117.77.7e-06428452119
     zf-NF-X1   1 CG......kHkCqklCHeGpCppCp 19 
                  CG      +HkCq++CH GpC pCp
  AT5G05660.1 428 CGkklrcrNHKCQSPCHQGPCAPCP 452
                  777777779***************9 PP

Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
PROSITE patternPS01359084148IPR019786Zinc finger, PHD-type, conserved site
CDDcd060088.27E-5183231No hitNo description
SMARTSM004388.1195213IPR000967Zinc finger, NF-X1-type
PfamPF014222.5195212IPR000967Zinc finger, NF-X1-type
CDDcd060085.05E-9237285No hitNo description
SMARTSM004383.5E-4247266IPR000967Zinc finger, NF-X1-type
PfamPF014225.0E-4247265IPR000967Zinc finger, NF-X1-type
CDDcd060088.02E-7290330No hitNo description
SMARTSM004380.05300319IPR000967Zinc finger, NF-X1-type
PfamPF014220.064300318IPR000967Zinc finger, NF-X1-type
CDDcd060082.18E-9344393No hitNo description
SMARTSM004380.035354374IPR000967Zinc finger, NF-X1-type
PfamPF014220.0013354373IPR000967Zinc finger, NF-X1-type
CDDcd060081.05E-6397445No hitNo description
SMARTSM004380.33407426IPR000967Zinc finger, NF-X1-type
PfamPF014220.036407425IPR000967Zinc finger, NF-X1-type
CDDcd060085.05E-11424472No hitNo description
PfamPF014227.0E-4428452IPR000967Zinc finger, NF-X1-type
SMARTSM004380.0034434453IPR000967Zinc finger, NF-X1-type
SMARTSM0043876491512IPR000967Zinc finger, NF-X1-type
PfamPF014224.8501511IPR000967Zinc finger, NF-X1-type
SMARTSM00438220520565IPR000967Zinc finger, NF-X1-type
PfamPF0142276520529IPR000967Zinc finger, NF-X1-type
SMARTSM0043833602633IPR000967Zinc finger, NF-X1-type
PfamPF014220.45602612IPR000967Zinc finger, NF-X1-type
SMARTSM004380.41643661IPR000967Zinc finger, NF-X1-type
PfamPF0142217644660IPR000967Zinc finger, NF-X1-type
SMARTSM0043848706735IPR000967Zinc finger, NF-X1-type
PfamPF014226.9708720IPR000967Zinc finger, NF-X1-type
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0000122Biological Processnegative regulation of transcription from RNA polymerase II promoter
GO:0007623Biological Processcircadian rhythm
GO:0009651Biological Processresponse to salt stress
GO:0009908Biological Processflower development
GO:0010310Biological Processregulation of hydrogen peroxide metabolic process
GO:0016567Biological Processprotein ubiquitination
GO:0042335Biological Processcuticle development
GO:0045892Biological Processnegative regulation of transcription, DNA-templated
GO:0045893Biological Processpositive regulation of transcription, DNA-templated
GO:2000037Biological Processregulation of stomatal complex patterning
GO:0005634Cellular Componentnucleus
GO:0016021Cellular Componentintegral component of membrane
GO:0000977Molecular FunctionRNA polymerase II regulatory region sequence-specific DNA binding
GO:0000987Molecular Functioncore promoter proximal region sequence-specific DNA binding
GO:0001078Molecular Functiontranscriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding
GO:0008270Molecular Functionzinc ion binding
GO:0016874Molecular Functionligase activity
Plant Ontology ? help Back to Top
PO Term PO Category PO Description
PO:0000013anatomycauline leaf
PO:0000037anatomyshoot apex
PO:0000084anatomyplant sperm cell
PO:0000230anatomyinflorescence meristem
PO:0000293anatomyguard cell
PO:0008019anatomyleaf lamina base
PO:0009005anatomyroot
PO:0009006anatomyshoot system
PO:0009009anatomyplant embryo
PO:0009010anatomyseed
PO:0009025anatomyvascular leaf
PO:0009029anatomystamen
PO:0009030anatomycarpel
PO:0009031anatomysepal
PO:0009032anatomypetal
PO:0009046anatomyflower
PO:0009047anatomystem
PO:0009052anatomyflower pedicel
PO:0020030anatomycotyledon
PO:0020038anatomypetiole
PO:0020100anatomyhypocotyl
PO:0020137anatomyleaf apex
PO:0025022anatomycollective leaf structure
PO:0025195anatomypollen tube cell
PO:0025281anatomypollen
PO:0001016developmental stageL mature pollen stage
PO:0001017developmental stageM germinated pollen stage
PO:0001054developmental stagevascular leaf senescent stage
PO:0001078developmental stageplant embryo cotyledonary stage
PO:0001081developmental stagemature plant embryo stage
PO:0001185developmental stageplant embryo globular stage
PO:0004507developmental stageplant embryo bilateral stage
PO:0007064developmental stageLP.12 twelve leaves visible stage
PO:0007095developmental stageLP.08 eight leaves visible stage
PO:0007098developmental stageLP.02 two leaves visible stage
PO:0007103developmental stageLP.10 ten leaves visible stage
PO:0007115developmental stageLP.04 four leaves visible stage
PO:0007123developmental stageLP.06 six leaves visible stage
PO:0007611developmental stagepetal differentiation and expansion stage
PO:0007616developmental stageflowering stage
Sequence ? help Back to Top
Protein Sequence    Length: 880 aa     Download sequence    Send to blast
MAGTATTEFR WKSPPQPPSQ EQPISDSDSD SGSDSENHQH RHNDLSNSIF EAYLDCHSSS  60
SPSSIDLAKI QSFLASSSSG AVSCLICLER IKRTDPTWSC TSSCFAVFHL FCIQSWARQC  120
LDLQAARAVT RPSSNPTEPE AVWNCPKCRS SYQKSKIPRR YLCYCGKEED PPADNPWILP  180
HSCGEVCERP LSNNCGHCCL LLCHPGPCAS CPKLVKAKCF CGGVEDVRRC GHKQFSCGDV  240
CERVLDCNIH NCREICHDGE CPPCRERAVY KCSCGKVKEE KDCCERVFRC EASCENMLNC  300
GKHVCERGCH AGECGLCPYQ GKRSCPCGKR FYQGLSCDVV APLCGGTCDK VLGCGYHRCP  360
ERCHRGPCLE TCRIVVTKSC RCGVTKKQVP CHQELACERK CQRVRDCARH ACRRRCCDGE  420
CPPCSEICGK KLRCRNHKCQ SPCHQGPCAP CPIMVTISCA CGETHFEVPC GTETNQKPPR  480
CRKLCHITPL CRHGQNQKPH KCHYGACPPC RLLCDEEYPC GHKCKLRCHG PRPPPNREFI  540
LKPTKKMLHI QAESTPGSPC PRCPEPVWRP CVGHHLAAEK RMICSDRTQF ACDNLCGNPL  600
PCGNHYCSYF CHALDIRSSS LDKRSESCEK CDLRCQKERT PRCQHPCPRR CHPEDCPPCK  660
TLVKRSCHCG AMVHAFECIY YNTMSEKDQM KARSCRGPCH RKLPNCTHLC PEICHPGQCP  720
LPEKCGKKVV VRCKCLTLKK EWVCQDVQAA HRATGSDPKE VPKNQFGVGL LPCDSNCKSK  780
LQVAESVLTQ RNVKEIEEKE EPSGKNASKR RKRRGRGQDI QETTRLQKLA VTTKRILMVV  840
MLVAMLAAVS YYGYKGLLWL SDWMNEVEEQ RQKSRRYPRI
Expression -- UniGene ? help Back to Top
UniGene ID E-value Expressed in
At.329700.0flower| leaf| root
Expression -- Microarray ? help Back to Top
Source ID E-value
GEO1865204070.0
Genevisible250767_at0.0
Expression AtlasAT5G05660-
AtGenExpressAT5G05660-
ATTED-IIAT5G05660-
Expression -- Description ? help Back to Top
Source Description
UniprotTISSUE SPECIFICITY: Constitutively expressed in mesophyll and guard cells. {ECO:0000269|PubMed:22073231}.
Functional Description ? help Back to Top
Source Description
TAIREncodes a homolog of the mammalian zinc finger transcription factor NF-X1.
UniProtProbable transcriptional regulator. May mediate E2- or E3-dependent ubiquitination. Required to gate light sensitivity during the night. Regulates the speed of the clock by acting in the feedback loop between CCA1, LHY and APRR1/TOC1. Promotes the expression of CCA1 at night but not by days. This activational effect is enhanced by interaction with ADO1/ZTL. Association with ADO1/ZTL is not leading to the degradation of NFXL2. Confers sensitivity to osmotic stress such as high salinity. Prevents H(2)O(2) production and abscisic acid accumulation. Part of a regulatory network that integrates the biosynthesis and action of abscisic acid, reactive oxygen species and cuticle components. {ECO:0000269|PubMed:16905136, ECO:0000269|PubMed:21300918, ECO:0000269|PubMed:21429190, ECO:0000269|PubMed:22073231, ECO:0000269|PubMed:22516817}.
Function -- GeneRIF ? help Back to Top
  1. The ebi-1 mutation alters the expression of core circadian clock components, and the ebi-1 mutant is early flowering.
    [PMID: 21300918]
  2. NFXL2-78 protein is part of a regulatory network that integrates the biosynthesis and action of ABA, ROS, and cuticle components.
    [PMID: 22516817]
Cis-element ? help Back to Top
SourceLink
PlantRegMapAT5G05660.1
Regulation -- Description ? help Back to Top
Source Description
UniProtINDUCTION: Circadian-regulation with a peak of expression at or before dawn. Not regulated by biotic and abiotic stresses, by light and nutrient conditions or upon treatment with elicitors, chemicals, abscisic acid or phytohormones. {ECO:0000269|PubMed:21300918, ECO:0000269|PubMed:22073231}.
Regulation -- PlantRegMap ? help Back to Top
Source Upstream Regulator Target Gene
PlantRegMapRetrieve-
Phenotype -- Disruption Phenotype ? help Back to Top
Source Description
UniProtDISRUPTION PHENOTYPE: No obvious morphological alterations. Enhanced growth and survival under water or salt stress. Enhanced H(2)O(2) production. Elevated abscisic acid levels and reduced stomatal aperture. {ECO:0000269|PubMed:16905136, ECO:0000269|PubMed:22073231}.
Phenotype -- Mutation ? help Back to Top
Source ID
T-DNA ExpressAT5G05660
Annotation -- Nucleotide ? help Back to Top
Source Hit ID E-value Description
GenBankAK2286900.0AK228690.1 Arabidopsis thaliana mRNA for hypothetical protein, clone: RAFL16-04-K08.
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqNP_196185.30.0NF-X1-type zinc finger protein NFXL2
SwissprotQ9FFK80.0NFXL2_ARATH; NF-X1-type zinc finger protein NFXL2
TrEMBLD7LYZ50.0D7LYZ5_ARALL; Transcription factor
STRINGAT5G05660.10.0(Arabidopsis thaliana)
Orthologous Group ? help Back to Top
LineageOrthologous Group IDTaxa NumberGene Number
MalvidsOGEM109542730
Representative plantOGRP63251718
Publications ? help Back to Top
  1. Lisso J,Altmann T,Müssig C
    The AtNFXL1 gene encodes a NF-X1 type zinc finger protein required for growth under salt stress.
    FEBS Lett., 2006. 580(20): p. 4851-6
    [PMID:16905136]
  2. Wang Y, et al.
    Transcriptome analyses show changes in gene expression to accompany pollen germination and tube growth in Arabidopsis.
    Plant Physiol., 2008. 148(3): p. 1201-11
    [PMID:18775970]
  3. M
    Structure and putative function of NFX1-like proteins in plants.
    Plant Biol (Stuttg), 2010. 12(3): p. 381-94
    [PMID:20522174]
  4. Johansson M, et al.
    Partners in time: EARLY BIRD associates with ZEITLUPE and regulates the speed of the Arabidopsis clock.
    Plant Physiol., 2011. 155(4): p. 2108-22
    [PMID:21300918]
  5. Ashelford K, et al.
    Full genome re-sequencing reveals a novel circadian clock mutation in Arabidopsis.
    Genome Biol., 2011. 12(3): p. R28
    [PMID:21429190]
  6. Lisso J,Schr
    NFX1-LIKE2 (NFXL2) suppresses abscisic acid accumulation and stomatal closure in Arabidopsis thaliana.
    PLoS ONE, 2011. 6(11): p. e26982
    [PMID:22073231]
  7. Lisso J,Schr
    NFXL2 modifies cuticle properties in Arabidopsis.
    Plant Signal Behav, 2012. 7(5): p. 551-5
    [PMID:22516817]